Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It often multiplicities via seed but barricaded F1 sterility. In vitro culture walleriana has much significant function in fast proliferation with useful features and elicitation healthful disease-free plants. experiment was conducted to investigate the impact medium different hormones on propagation using a completely randomized design. MS prepared along various concentrations BAP, TDZ ZEA. Callus induced grew well media supplemented 0.5 mg/l NAA + 1 BAP. order indirect propagation, explants were cultured same containing ZEA combination NAA. These treatments have ability organogenesis. The results revealed that control treatment had lowest effect traits including shoot percentage, number shoots, leaves, length, fresh dry weight, it lead maximum proliferations highest root length rooting percentage observed IBA addition, mutation agents studied. Aseptic samples treated ?- irradiation, Ethyl Methane Sulfonate colchicines at growth chamber. Treatments 30 60 grey respectively lower survival rate, rate polyploidy while 0.1 0.2 rats. Regarding these, present technique illustrate effective system for reproduction hypocotyls cultures. proved be induction this plantlet.